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Industrial Training and
Project Work/
Dissertation Work
Starting from 3rd Jan 2011
onwards

            on

Fermentation Technology

             &

           Water

Purification Technology

             for

M.Sc.,B.Sc.,B.Sc.,(Hons),B.E.,B.Tech.

(Biotechnology/Biochemistry/

Microbiology)Students/

Industrial Professionals

              &

           Faculty of

Universities/Institutes/

            Colleges.

for Registration and Training

request for the Prospectus available

at Bio-Age Office Mohali (Pb.) India.

 

 
 
Training Modules/Curriculum      
 

TRAINING  MODULES 

1. TWO WEEKS TRAINING MODULE

WEEK 1:Theory:-General Biotechnology, Microbiology, Microscopy and Circumscription of a taxon.

Practicals:-
(A) Instrumentation§         UV/Visible Spectrophotometers§         Laminar air flow§         Biosafety cabinets§         Microscopes:a.       compound b.      binocularc.       trinocular§         pH meters§         Autoclaves§         Refrigerated centrifuge§         Shakers§         Water Purification systems§         Auto Pipettes§         Weighing balances     (B) Microscopy and slide preparation
  • Simple staining
  • Gram staining
  • Fungal staining           
 (C)  Shake Flask Fermentation
  • Standard curve preparation for reducing sugar/s estimation by DNS method.
  • Standard curve preparation for Ethanol estimation by  Potassium dichromate method.
  • Concept of sterilization:
a) Media preparation for cultivation of Yeast/Bacteria.b) Sterilization of Media.c) Inoculation under aseptic conditions.
  • Sampling and Analysis for substrate utilization and product estimation.
  • Compilation of data; Calculation of:
(a) Residual Sugar(b)   Ethanol Production(c)    Fermentation efficiency 

WEEK 2:

Theory:-Fermenter/Biorector design and its parts(Autoclavable Type).
         Practicals:-
(A)  Working of Fermenter       (Autoclavable Type)§         Preparation of media for cultivation  of Yeast/Bacteria.§         Leak test for bioreactor. §         Sterilization of media and bioreactor by Autoclaving.§         Inoculation under aseptic       conditions.(C) Product Harvesting. (E) Verification and submission of       Report.    (B)   Sampling and Analysis for determination of: §          Growth§          Residual substrate§          Product estimation§          Cell count/ml§          pH-variation  (D  Compilation of data;Calculation of:(a) Residual sugar/s(b)Ethanol Production   (c) Fermentation efficiency

2.  FOUR WEEKS TRAINING MODULE

WEEK 1:Theory:-General Biotechnology, Microbiology, Microscopy and Circumscription of a taxon.
Practicals:-
(A) Instrumentation§         UV/Visible Spectrophotometer§         Laminar air flow§         Biosafety cabinets§         Microscopes:(a) compound (b) binocular(c) trinocular§         pH meter§         Autoclaves§         Refrigerated centrifuge§         Shakers§         Water purification systems§         Auto pipettes§         Weighing balances§         Filtration Techniques     (B) Microscopy and slide preparation
  • Simple staining
  • Gram staining
  • Fungal staining           
     (C)  Shake Flask Fermentation:Total Sugar
  • Standard curve preparation for reducing sugar/s estimation by DNS method.
  • Standard curve preparation for Ethanol estimation by  Potassium dichromate method.
  • Concept of sterilization :
a)Media preparation for cultivation of Yeast/      Bacteria.b)Sterilization of Media.c)Inoculation under aseptic conditions.
  • Sampling and Analysis for substrate utilization and product estimation.
  • Compilation of data; Calculation of:
(a) Residual Sugar(b) Ethanol Production(c) Fermentation efficiency
WEEK 2:Theory:-Fermenter/Bioreactor design and its parts(Autoclavable Type).
Practicals:-
(A)Working of Fermenter       (Autoclavable Type)§         Preparation of media for        Cultivation of Yeast/Bacteria.§         Leak test for fermenter. §         Sterilization of media and       fermenter by Autoclaving.§         Inoculation under aseptic      condition. (C) Product Harvesting.   (B) Sampling and Analysis for determination  of:§         Growth§        Residual substrate§        Product estimation§        Cell count/ml§        pH-variation   (D) Compilation of data; Calculation of:       (a) Residual sugar/s (b) Ethanol Production                         (c) Fermentation efficiency.
WEEK 3:   Theory:-
(A)  Major Water Contaminants
  • Suspended particulate, silt, organic materials.
  • Dissolved particulates organic/inorganics
  • Bacteria/Viruses/Algae
  • Pyrogens/RNase/DNase/ Endotoxins.
 (C) Membrane filtration§         Laboratory/industrial use of MF§         Manufacturing process of membrane§         Characteristics of membrane and Prefilters. 		   B) Ionic impurities in water purification     Techniques:§        Sedimentation§        Carbon filtration/Disinfection by        chlorine/UV.§        Deionisation(Resins/EDI)§        Distillation/single/double/triple§        Filtration(RO/UF Media).   (D) Types of Membranes/MOC’s§        Microfilteration§        Ultrafiltration§        Nano filtration§        Reverse Osmosis§        Role of R.O. technology in water      and  product purification.  
Practicals:-
(A) Water Testing:Determination of total count of coliform in ground water, effluent water.    (a) MPN Method          (b) MF Method   (B) Isolation of microorganisms capable of producing commercially important enzymes. 
WEEK 4:Theory:-
(A) Solid State Fermentation.            (C) Enzyme production and their      Assays.   (B) Submerged Fermentation  
Practicals:-
(A)Fermentation Batch Type (Shake flask)§        Solid State Fermentation.§        Submerged Fermentation§        Enzyme Assays.:      (a) ά-amylase (b) CMCase(C) Industrial Visit.      (B) Compilation of data;Calculation of:(a) Residual sugar/s(b) Ethanol Production(c) Fermentation efficiency.  (D) Verification and submission of Report.

3.  SIX WEEKS TRAINING MODULE

WEEK 1:Theory:-General Biotechnology, Microbiology, Microscopy and Circumscription of a taxon.
Practicals:-
(A) Instrumentation§         HPLC§         UV/Visible Spectrophotometer§         Laminar air flow§         Biosafety cabinets§         Microscopes:(a) compound (b) binocular(c) trinocular§         pH meter§         Autoclaves§         Refrigerated centrifuge§         Shakers§         Water purification system§         Auto pipettes§         Weighing balances§         Filtration Techniques     (B) Microscopy and slide preparation§         Simple staining§         Gram staining§         Fungal staining §         Spore staining§         Dark field microscopy           (C)  Shake Flask Fermentation:Total Sugar§   Standard curve preparation for reducing sugar estimation by DNS method.§   Standard curve preparation for Ethanol estimation by  Potassium dichromate method.§   Concept of sterilization :-a) Different media preparation for  cultivation of Yeast/Bacteria and fungi.b) Sterilization of Media.c) Inoculation under aseptic conditions.§   Sampling and Analysis for substrate utilization and product estimation.§   Compilation of data; Calculation of:(a) Residual Sugar(b) Ethanol Production      (c) Fermentation efficiency.
WEEK 2:Theory:-Fermenter/Bioreactor Design and its parts (Autoclavable Type).
Practicals:-
(A)Working of Fermenter      (Autoclavable Type)§         Preparation of media,       cultivation of Yeast/Bacteria.§         Leak test for Bioreactor. §         Sterilization of media and      bioreactor by Autoclaving.§         Inoculation under aseptic       conditions. (C) Product Harvesting.    (B) Sampling and Analysis for determination of:§         Growth§        Residual substrate§        Product estimation§        Cell count/ml§        pH-variation    (D Compilation of data; Calculation of:(a)  Residual Sugar(b) Ethanol Production(c) Fermentation efficiency.  
WEEK 3:Theory:-
(A)  Major Water Contaminants
  • Suspended particulate, silt, organic materials.
  • Dissolved particulates organic/inorganics
  • Bacteria/ Viruses/Algae
  • Pyrogens/RNase/DNase/Endotoxins.
(C) Membrane filtration§   Laboratory/industrial use of MF§   Manufacturing process of membrane§   Characteristics of membrane and Prefilters. 		   (B) Ionic impurities in water purificationTechniques:§         Sedimentation§         Carbon filtration/Disinfection by        chlorine/UV§         Deionisation(Resins/EDI)§         Distillation/single/double/triple§         Filtration(RO/UF Media).   (D) Types of Membrane/MOC’s§        Microfilteration §        Ultrafiltration§        Nano filtration§        Reverse Osmosis§        Role of R.O technology in water      and  product purification.  
Practicals:-
(A) Water testing:Determination of total count of coliform in ground water, effluent water.    (a) MPN Method    (b) MF Method    (B)Isolation of microorganisms capable of      producing commercially important enzymes. 
WEEK 4:Theory:-
(A) Solid State Fermentation.(C) Enzyme production and their        Assays.   (B) Submerged Fermentation. 
WEEK 4:Practicals:
(A)Fermentation Batch Type (Shake flask)§        Solid State Fermentation.§        Submerged Fermentation§        Enzyme Assays.:      (a) ά-amylase (b) CMCase(C)   Industrial Visit.    (B) Compilation of data;Calculation of:-(a) Residual sugar/s(b) Ethanol Production(c) Fermentation efficiency.  
WEEK 5:Theory:- Down stream Processing Techniques:
(A) Cell distruption  Methods§         Physical§         Chemical§         Mechanical (C)Determination of purity and        molecular weight of protein.    (B) Chromatographics Techniques §        Ion exchange §        Gel filtration methods§        Hydrophobic Interaction §        Bioaffinity§        Covalent§        HPLC§        GC 
Practicals:- Protein concentration techniques:
(A) Cell disruption Methods§         Physical§         Chemical(C)Biochemical characterization of microorganisms.   B)Electrophoresis:PAGE/SDS PAGE 
WEEK 6 Theory:-Fermenter/Biorector design and its  parts (In–situ Type).
Practicals:
1. (A) Working of In- situ fermenter§         Media preparation          cultivation of yeast/Bacteria.§         Sterilization Media and          fermenter through steam.§         Dry run for sterility test.§         Inoculation under aseptic          Conditions.(A)  Product harvesting.(E) Enzyme Assays:- (i) Glucoamylase   (ii) FPase     (B) Sampling and analysis at regular   interval of time for the determination of:   §               Growth §               Cell count§               pH-variation§               Residual Substrate §               Product estimation  (D) Compilation of data;calculation of:(a) Residual Sugar(b) Ethanol(c)  Fermentation efficiency.
2. (A)Theoretical concept and     Practical exposure on HPLC.  (B)   Verification and       Submission of Reports.    (B)  Industrial Visit.   

NOTE:

Prior company presentation is arranged in the respective college/s /Universities/Institutes for interested students on request basis as per the convenience of HOD/Principal/Director.

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